ABSTRACT
Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester (CHS) ,one of the raw materials .Methods CHS was purified by recrystallization ,the pure CHS structure was characterizate by differential scanning calorimetry (DSC) and X ray diffraction (XRD) ,the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography (HPLC) ,the degree of substitution of the cholesteryl was determina-ted before and after the purified by 1 H-NMR .Result CHS was purer after recrystallization ,with property of crystal ,which could significantly improve the degree of substitution of the cholesteryl .Conclusion The method of purification was simple and feasible ,which was the basis of improving hydrophobically modified Bletilla striata polysaccharide′s degree of substitution of the cholesteryl .